The Production of Cephalosporin C by Acremonium Chrysogenum Is Improved by the Intracellular Expression of a Bacterial Hemoglobin

Biotechnology (N Y). 1993 Aug;11(8):926-9. doi: 10.1038/nbt0893-926.


A DNA vector for expressing an oxygen-binding heme protein (Vitreoscilla hemoglobin, or VHb) in filamentous fungi was constructed and introduced into a cephalosporin C-producing strain of Acremonium chrysogenum. Expression of VHb in transformants was demonstrated by Western immunoblot analysis and by increased carbon monoxide binding activity of cell extracts. Several VHb-expressing transformants produced significantly higher yields of cephalosporin C than control strains in batch culture experiments. Using the same vector system, VHb was also expressed in the related fungus Penicillium chrysogenum.

MeSH terms

  • Acremonium / genetics
  • Acremonium / metabolism*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Blotting, Northern
  • Blotting, Western
  • Carbon Monoxide / metabolism
  • Cephalosporins / biosynthesis*
  • Chromatography, High Pressure Liquid
  • Gene Expression*
  • Hemoglobins / genetics*
  • Hemoglobins / metabolism
  • Plasmids
  • Transfection
  • Truncated Hemoglobins


  • Bacterial Proteins
  • Cephalosporins
  • Hemoglobins
  • Truncated Hemoglobins
  • hemoglobin protein, Vitreoscilla
  • cephalosporin C
  • Carbon Monoxide