A study of process interactions between cell disruption and debris clarification stages in the recovery of yeast intracellular products

Biotechnol Prog. Sep-Oct 1993;9(5):462-7. doi: 10.1021/bp00023a004.


The effects of varying the levels of cell disruption achieved by high-pressure homogenization upon subsequent centrifugal separation of cell debris have been defined by measuring sedimentation velocity distributions of the cell debris. Using this data together with particle size distributions, a computer-based model of a disk stack centrifuge has been used to predict the efficiency of centrifugal separation. These predictions have been compared with both laboratory scale and pilot plant scale experimental results. A similar sedimentation velocity technique has been used to characterize the properties of the flocs formed by addition of poly(ethylene imine) to a borax-clarified homogenate supernatant. The variations of floc settling properties with respect to polymer concentration are defined.

MeSH terms

  • Biotechnology / methods*
  • Cell Separation / methods
  • Centrifugation / methods
  • Computer Simulation
  • Flocculation
  • Fungal Proteins / isolation & purification*
  • Intracellular Fluid / chemistry
  • Intracellular Fluid / metabolism
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae / metabolism


  • Fungal Proteins