Simple method for extracting plasmid DNA from lactic acid bacteria

Lett Appl Microbiol. 1994 Apr;18(4):227-9. doi: 10.1111/j.1472-765x.1994.tb00853.x.

Abstract

Rapid screening and large-scale plasmid DNA isolation procedures are described for lactic acid bacteria, using glass beads to break cells. The rapid screening procedure allows one to obtain plasmid DNA pellets in less than 1 h. This method has been successfully tested on various bacteria from the genera Lactococcus, Leuconostoc, Lactobacillus, Pediococcus, Streptococcus, Enterococcus and Propionibacterium. This procedure yields plasmid DNA with minor chromosomal and plasmid DNA-degraded form contaminations.

MeSH terms

  • Bacteriological Techniques*
  • Biotechnology
  • Enterococcus / chemistry
  • Evaluation Studies as Topic
  • Gram-Positive Bacteria / chemistry*
  • Gram-Positive Bacteria / metabolism
  • Lactates / metabolism*
  • Lactic Acid
  • Lactobacillus / chemistry
  • Lactococcus / chemistry
  • Leuconostoc / chemistry
  • Pediococcus / chemistry
  • Plasmids / isolation & purification*
  • Propionibacterium / chemistry
  • Streptococcus / chemistry

Substances

  • Lactates
  • Lactic Acid