Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 May 15;34(2):185-93.
doi: 10.1016/0168-1656(94)90088-4.

Rapid and Quantitative Analysis of Recombinant Protein Expression Using Pyrolysis Mass Spectrometry and Artificial Neural Networks: Application to Mammalian Cytochrome b5 in Escherichia Coli

Affiliations

Rapid and Quantitative Analysis of Recombinant Protein Expression Using Pyrolysis Mass Spectrometry and Artificial Neural Networks: Application to Mammalian Cytochrome b5 in Escherichia Coli

R Goodacre et al. J Biotechnol. .

Abstract

Recombinant Escherichia coli clones encoding between 0 and 6 copies of the mammalian cytochrome b5 gene were subjected to pyrolysis mass spectrometry (PyMS). To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the amount of cytochrome b5 produced fully-interconnected feedforward artificial neural networks (ANNs) were studied. It was found that the combination of PyMS and ANNs could be used to predict the amount of cytochrome b5 expressed in E. coli. PyMS is a novel, convenient and rapid method for the screening and analysis of microbial and other cultures producing recombinant proteins.

Similar articles

See all similar articles

Cited by 3 articles

Publication types

LinkOut - more resources

Feedback