A rapid method for measuring organelle-specific substrate transport in homogenates from plant tissues

Planta. 1994;194(2):181-5.

Abstract

A rapid method is described for measuring organelle-specific metabolite transport systems in crude homogenates from plants. The tissues were homogenized in liquid nitrogen, extracted with buffer and reconstituted into artificial membranes. The method allowed demonstration of the known different substrate specificities of chloroplast triose phosphate/phosphate translocators from C3- and C4-plants, of the triose phosphate/phosphate translocator from non-green tissue, and of the dicarboxylate translocator. It thus by-passes the necessity to isolate intact plant organelles and, in addition, only a low amount of tissue material is required for transport measurements.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Carbon Radioisotopes
  • Cell Membrane / metabolism
  • Chloroplasts / metabolism
  • Liposomes
  • Malates / metabolism
  • Organelles / metabolism*
  • Phosphates / metabolism
  • Phosphorus Radioisotopes
  • Plants / metabolism*
  • Species Specificity
  • Sugar Phosphates / metabolism

Substances

  • Carbon Radioisotopes
  • Liposomes
  • Malates
  • Phosphates
  • Phosphorus Radioisotopes
  • Sugar Phosphates