Purification of an acidic coniferin-hydrolysing beta-glucosidase from developing xylem of Pinus banksiana

V Leinhos; P V Udagama-Randeniya; R A Savidge

doi:10.1016/0031-9422(94)85053-4

Purification of an acidic coniferin-hydrolysing beta-glucosidase from developing xylem of Pinus banksiana

Phytochemistry. 1994 Sep;37(2):311-5. doi: 10.1016/0031-9422(94)85053-4.

Authors

V Leinhos¹, P V Udagama-Randeniya, R A Savidge

Affiliation

¹ FSU Jena, Institut für Ernährung und Umwelt, Germany.

PMID: 7765617
DOI: 10.1016/0031-9422(94)85053-4

Abstract

Two cytosolic, acidic (pI 3.8) glycoproteins (M(r) 110,000 and 90,000) from lignifying xylem of Pinus banksiana were electrophoretically isolated and confirmed by combined gas chromatography-mass spectrometry to be capable of hydrolysing E-coniferin to trans-coniferyl alcohol. These isoenzymes yielded identical N-terminal sequences having negligible homology with documented glucosidases, suggesting that they constitute a novel form of beta-glucosidase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Chromatography, Ion Exchange
Cinnamates / metabolism*
Hydrolysis
Isoelectric Focusing
Molecular Sequence Data
Trees / enzymology*
Trees / growth & development
beta-Glucosidase / isolation & purification*
beta-Glucosidase / metabolism

Substances

Cinnamates
beta-Glucosidase
coniferin