Construction of Pta-Ack pathway deletion mutants of Escherichia coli and characteristic growth profiles of the mutants in a rich medium

Biosci Biotechnol Biochem. 1994 Dec;58(12):2232-5. doi: 10.1271/bbb.58.2232.


Escherichia coli grown in a rich medium excreted acetate and reused the acetate. Using cloned genes and a plasmid with a temperature-sensitive replication origin, three kinds of Pta-Ack pathway deletion mutants were constructed. Acetate production and reuse by wild-type cells grown in the rich medium was confirmed to largely occur through the Pta-Ack pathway. The deletion mutants of the gene encoding phosphotransacetylase secreted pyruvate before the secretion of acetate into the medium. A deletion mutant of the gene encoding acetate kinase grew at a slow rate, but its secretion and use of acetate were rapid. These results indicated that a pathway(s), other than the Pta-Ack pathway, functions in the control of excess carbon flow in the mutants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetate Kinase / genetics
  • Acetate Kinase / metabolism*
  • Acetates / metabolism
  • Acetic Acid
  • Cloning, Molecular
  • Culture Media
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli / growth & development
  • Mutation
  • Phosphate Acetyltransferase / genetics
  • Phosphate Acetyltransferase / metabolism*
  • Sequence Deletion


  • Acetates
  • Culture Media
  • Phosphate Acetyltransferase
  • Acetate Kinase
  • Acetic Acid