Metabolism of dodecyldimethylamine by Pseudomonas MA3

Appl Microbiol Biotechnol. 1994 Oct;42(1):134-9. doi: 10.1007/BF00170236.

Abstract

Pseudomonas MA3 was isolated from activated sludge on the basis of its capacity to use dodecyldimethylamine as a sole carbon (C) and energy source. Dodecylamine, dodecanal, dodecanoic acid and acetic acid also supported growth of Pseudomonas MA3. Dodecyldimethylamine-grown cells oxidized a wide range of alkylamine derivatives, dodecanal, dodecanoic acid and acetic acid. Degradation of the alkyl chain of dodecyldimethylamine by Pseudomonas MA3 appeared from the stoichiometric liberation of dimethylamine. A dehydrogenase catalysed the cleavage of the Calkyl-N bond. The first intermediate of the proposed degradation pathway, dodecanal, accumulated in the presence of decanal used as a competitive inhibitor. The second intermediate, dodecanoic acid, was formed in the presence of acrylic acid during the degradation of dodecyldimethylamine. Dodecanal was converted into dodecanoic acid by a dehydrogenase and dodecanoic acid was then degraded via the beta oxidation pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / metabolism*
  • Biodegradation, Environmental
  • Biotechnology
  • Environmental Pollutants / metabolism
  • Models, Chemical
  • Oxidation-Reduction
  • Oxygen Consumption
  • Pseudomonas / growth & development
  • Pseudomonas / metabolism*
  • Surface-Active Agents / metabolism*

Substances

  • Amines
  • Environmental Pollutants
  • Surface-Active Agents
  • N,N-dimethyl-1-dodecanamine