A catechol 2,3-dioxygenase gene as a reporter

Biosci Biotechnol Biochem. 1995 Feb;59(2):314-5. doi: 10.1271/bbb.59.314.

Abstract

A catechol 2,3-dioxygenase (C23O) gene of Pseudomonas aeruginosa was expressed under the Simian virus 40 or Rous sarcoma virus promoter in mammalian cells; it was found that the gene could be used as a reporter for the study of gene expression. The C23O gene was a more sensitive reporter than the generally used beta-galactosidase gene.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Avian Sarcoma Viruses / genetics
  • Base Sequence
  • Catechol 2,3-Dioxygenase
  • DNA / chemistry
  • DNA / genetics
  • Dioxygenases*
  • Gene Expression Regulation, Enzymologic / genetics*
  • Genes, Bacterial
  • Genes, Reporter / genetics
  • Molecular Sequence Data
  • Oxygenases / genetics*
  • Plasmids
  • Promoter Regions, Genetic
  • Pseudomonas aeruginosa / enzymology*
  • Pseudomonas aeruginosa / genetics
  • Simian virus 40 / genetics
  • beta-Galactosidase / genetics

Substances

  • DNA
  • Oxygenases
  • Dioxygenases
  • Catechol 2,3-Dioxygenase
  • beta-Galactosidase

Associated data

  • GENBANK/X60740