Abstract
A catechol 2,3-dioxygenase (C23O) gene of Pseudomonas aeruginosa was expressed under the Simian virus 40 or Rous sarcoma virus promoter in mammalian cells; it was found that the gene could be used as a reporter for the study of gene expression. The C23O gene was a more sensitive reporter than the generally used beta-galactosidase gene.
MeSH terms
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Amino Acid Sequence
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Avian Sarcoma Viruses / genetics
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Base Sequence
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Catechol 2,3-Dioxygenase
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DNA / chemistry
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DNA / genetics
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Dioxygenases*
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Gene Expression Regulation, Enzymologic / genetics*
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Genes, Bacterial
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Genes, Reporter / genetics
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Molecular Sequence Data
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Oxygenases / genetics*
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Plasmids
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Promoter Regions, Genetic
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Pseudomonas aeruginosa / enzymology*
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Pseudomonas aeruginosa / genetics
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Simian virus 40 / genetics
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beta-Galactosidase / genetics
Substances
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DNA
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Oxygenases
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Dioxygenases
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Catechol 2,3-Dioxygenase
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beta-Galactosidase