Dopamine D3 receptor antisense influences dopamine synthesis in rat brain

Neuroreport. 1995 Feb 15;6(3):573-6. doi: 10.1097/00001756-199502000-00041.


Intracerebroventricular infusion of an all-phosphorothioate antisense oligodeoxynucleotide targeted at the rat dopamine D3 receptor mRNA (10 micrograms h-1, 5 days) resulted in a significant reduction (19%) of the binding of the [D2 + D3] ligand [3H]spiperone in the limbic forebrain, where D3 receptors are relatively abundant, but not in caudate-putamen, where D3 receptors are sparse. In nucleus accumbens antisense treatment also caused an increase in dopamine synthesis; in contrast, antisense administration did not counteract the effect of apomorphine on dopamine formation. No effect of antisense administration on dopamine synthesis was observed in caudate-putamen. The results support the usefulness of the antisense strategy for the study of D3 receptors and suggest that D3 receptors may influence dopamine synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antisense Elements (Genetics) / drug effects
  • Apomorphine / pharmacology
  • Brain / physiology*
  • Dopamine / biosynthesis*
  • Male
  • Nucleus Accumbens / physiology
  • Oligonucleotides, Antisense / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Dopamine / physiology*
  • Research Design
  • Spiperone / pharmacology
  • Substantia Nigra / physiology


  • Antisense Elements (Genetics)
  • Oligonucleotides, Antisense
  • Receptors, Dopamine
  • Spiperone
  • Apomorphine
  • Dopamine