Elevated levels of eukaryotic translation initiation factor eIF-4E, mRNA in a broad spectrum of transformed cell lines

Y Miyagi; A Sugiyama; A Asai; T Okazaki; Y Kuchino; S J Kerr

doi:10.1016/0304-3835(95)03737-h

Elevated levels of eukaryotic translation initiation factor eIF-4E, mRNA in a broad spectrum of transformed cell lines

Cancer Lett. 1995 May 8;91(2):247-52. doi: 10.1016/0304-3835(95)03737-h.

Authors

Y Miyagi¹, A Sugiyama, A Asai, T Okazaki, Y Kuchino, S J Kerr

Affiliation

¹ Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.

PMID: 7767916
DOI: 10.1016/0304-3835(95)03737-h

Abstract

Translation initiation factor eIF-4E, which binds to the 5' cap structure of eukaryotic mRNAs, is believed to play an important role in the control of cell growth. Over-expression of eIF-4E in fibroblasts results in their malignant transformation. However, no information on eIF-4E expression in established transformed cell lines has been available. We report here that a variety of tumor cell lines, chemically, virally and oncogenically transformed, exhibit elevated levels of eIF-4E mRNA expression as compared to their normal counterparts. Overexpression of eIF-4E, which is normally rate-limiting in protein synthesis, may stimulate the translation of regulatory and oncogenic proteins involved in transformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cell Line
Cell Transformation, Neoplastic*
DNA Primers / chemistry
Eukaryotic Initiation Factor-4E
Gene Expression Regulation, Neoplastic*
In Vitro Techniques
Molecular Sequence Data
Peptide Initiation Factors / genetics*
RNA, Messenger / genetics
RNA, Neoplasm / genetics
Rats

Substances

DNA Primers
Eukaryotic Initiation Factor-4E
Peptide Initiation Factors
RNA, Messenger
RNA, Neoplasm