Interleukin-10 expression and chemokine regulation during the evolution of murine type II collagen-induced arthritis

J Clin Invest. 1995 Jun;95(6):2868-76. doi: 10.1172/JCI117993.


In the enclosed study we have examined the expression and contribution of specific chemokines, macrophage inflammatory protein 1 alpha (MIP-1 alpha) and macrophage inflammatory protein 2 (MIP-2), and interleukin 10 (IL-10) during the evolution of type II collagen-induced arthritis (CIA). Detectable levels of chemotactic cytokine protein for MIP-1 alpha and MIP-2 were first observed between days 32 and 36, after initial type II collagen challenge, while increases in IL-10 were found between days 36 and 44. CIA mice passively immunized with antibodies directed against either MIP-1 alpha or MIP-2 demonstrated a delay in the onset of arthritis and a reduction of the severity of arthritis. On the contrary, CIA mice receiving neutralizing anti-IL-10 antibodies demonstrated an acceleration of the onset and an increase in the severity of arthritis. Interestingly, anti-IL-10 treatment increased the expression of MIP-1 alpha and MIP-2, as well as increased myeloperoxidase (MPO) activity and leukocyte infiltration in the inflamed joints. These data suggest that MIP-1 alpha and MIP-2 play a crucial role in the initiation and maintenance, while IL-10 appears to play a regulatory role during the development of experimental arthritis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arthritis, Experimental / metabolism*
  • Arthritis, Experimental / pathology
  • Base Sequence
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CXCL2
  • Chemotactic Factors / metabolism
  • Collagen / immunology*
  • Cytokines / metabolism*
  • DNA Primers / chemistry
  • Gene Expression
  • Immunization, Passive
  • Interleukin-10 / genetics
  • Interleukin-10 / metabolism*
  • Macrophage Inflammatory Proteins
  • Male
  • Mice
  • Mice, Inbred DBA
  • Molecular Sequence Data
  • Monokines / metabolism*
  • RNA, Messenger / genetics
  • Time Factors


  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CXCL2
  • Chemotactic Factors
  • Cytokines
  • DNA Primers
  • Macrophage Inflammatory Proteins
  • Monokines
  • RNA, Messenger
  • Interleukin-10
  • Collagen