Assessment of platelet cytosolic concentration of free magnesium in healthy subjects

J Lab Clin Med. 1995 Jun;125(6):743-7.

Abstract

We measured basal cytosolic concentration of free Mg2+ ([Mg2+]i) in platelets from 30 healthy volunteers by using mag-fura-2, a new fluorescent Mg2+ indicator. The mean [Mg2+]i was 381 +/- 22 mumol/L, with values ranging from 226 to 771 mumol/L. The day-to-day intrasubject coefficient of variation was relatively small (3.6%). [Mg2+]i was significantly higher in men than in women (430 +/- 38 vs 332 +/- 17 mumol/L, p < 0.03), and was not correlated with age. There was no significant relation between platelet [Mg2+]i and serum total Mg concentration. The results indicate that the gender but not age may decide intracellular Mg2+ status. In addition, the mechanisms that regulate [Mg2+]i may be independent of those that influence the extracellular concentration of Mg.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Age Factors
  • Aged
  • Aging / blood
  • Blood Platelets / chemistry
  • Blood Platelets / metabolism*
  • Cytosol / chemistry
  • Cytosol / metabolism
  • Female
  • Fluorescent Dyes
  • Fura-2 / analogs & derivatives
  • Humans
  • Magnesium / blood*
  • Male
  • Middle Aged
  • Reference Values
  • Sex Differentiation
  • Sex Factors

Substances

  • Fluorescent Dyes
  • 2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid
  • Magnesium
  • Fura-2