In vitro spheroplast and L-form induction within the pathogenic nocardiae

J Bacteriol. 1976 Jul;127(1):584-94. doi: 10.1128/jb.127.1.584-594.1976.


Six strains of Nocardia asteroides, two strains of N. caviae, and two strains of N. braziliensis were grown in medium supplementted with glycine, lysozyme, D-cycloserine, glycine plus lysozyme, and glycine plus D-cycloserine. It was shown that three strains of N. asteroides, and two strains of N. caviae, readily formed spheroplasts and/or protoplasts when grown in the presence of glycine plus either lysozyme or D-cycloserine. This process was studied by both phase contrast microscopy and electron microscopy. The induced cultures were then plated on hypertonic medium for the isolation of L-forms. It was shown that the organisms differed greatly in their ability to produce spheroplasts and subsequently grew as L-forms or transitional-phase variants.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Culture Media
  • Cycloserine / pharmacology
  • Glycine / pharmacology
  • L Forms / isolation & purification
  • L Forms / ultrastructure*
  • Muramidase / pharmacology
  • Nocardia / growth & development
  • Nocardia / isolation & purification
  • Nocardia / ultrastructure*
  • Nocardia asteroides / growth & development
  • Nocardia asteroides / isolation & purification
  • Nocardia asteroides / ultrastructure
  • Spheroplasts / isolation & purification
  • Spheroplasts / ultrastructure*


  • Culture Media
  • Cycloserine
  • Muramidase
  • Glycine