A correlation between the expression of the bcr-abl chimeric gene and severity of the clinical state of CML patients with time

Scand J Immunol. 1995 Jun;41(6):529-33. doi: 10.1111/j.1365-3083.1995.tb03603.x.


Using the reverse polymerase chain reaction, four Ph' positive CML patients were followed for 2 years; a correlation between the severity of the clinical state and the b3a2 expression was noted with time. Additionally, amplification of the c-myc proto-oncogene was observed, using Southern blot analysis, in one patient prior to his entry to the blast phase. No reorganization of the bcr-abl rearrangement site was found in the latter patient. The data suggest that a routine follow-up of CML patients using the Southern blot analysis and the reverse transcription-polymerase chain reaction might be of importance in evaluating the progression of the disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Blotting, Southern
  • Cytogenetics
  • DNA, Neoplasm / analysis
  • Female
  • Fusion Proteins, bcr-abl / biosynthesis
  • Fusion Proteins, bcr-abl / genetics*
  • Humans
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Severity of Illness Index


  • DNA, Neoplasm
  • Fusion Proteins, bcr-abl