Aphidicolin inhibits in vitro the activity of pseudorabies virus (PRV) DNA polymerase and in vivo the viral proliferation

In Vivo. 1994 Nov-Dec;8(6):1041-6.


We have purified and characterized Pseudorabies virus (PRV) DNA polymerase from infected TK- mouse cells. PRV DNA polymerase has a 3'- > 5' exonuclease activity; it is stimulated by ionic strength, requires magnesium for optimal activity and it is more sensitive to aphidicolin than eukaryotic and HSV-1 replicative DNA polymerases. Aphidicolin inhibits in vitro PRV DNA polymerase competitively with respect to dCTP with a Ki of 0.06 microM and completely blocks viral growth in vivo at 4.4 microM. The high sensitivity to aphidicolin of animal herpesvirus DNA polymerases might allow a topical use of this drug in the treatment of animal herpesvirus keratitis and stomatitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphidicolin / pharmacology*
  • Cell Line
  • Herpesvirus 1, Suid / drug effects
  • Herpesvirus 1, Suid / enzymology*
  • Herpesvirus 1, Suid / physiology
  • Mice
  • Nucleic Acid Synthesis Inhibitors*
  • Virus Replication / drug effects


  • Nucleic Acid Synthesis Inhibitors
  • Aphidicolin