Nucleotide sequence analysis of the carbomycin biosynthetic genes including the 3-O-acyltransferase gene from Streptomyces thermotolerans

Biosci Biotechnol Biochem. 1995 Apr;59(4):582-8. doi: 10.1271/bbb.59.582.

Abstract

A 3.2-kb DNA fragment of the carbomycin biosynthetic region including the 3-O-acyltransferase gene (acyA) from Streptomyces thermotolerans was sequenced, and four ORFs were found in the fragment. The second ORF, designated ORF-A, was transcribed in the opposite direction to the other three ORFs. The first ORF was identified as carA, a gene for carbomycin resistance. The amino acid sequence of ORF-A was homologous to proteins of the cytochrome P-450 family. Streptomyces lividans transformed with pCB20, in which ORF-A was subcloned, epoxidized carbomycin B at its C-12, 13 positions, thus producing carbomycin A. The third ORF, the amino acid sequence of which showed a homology to macrolide antibiotics O-acyltransferases was identified as acyA. The last ORF (ORF-B), which starts just 3 bp downstream from the TGA termination codon of acyA, was thought to be a carbomycin 4-O-methyltransferase gene, because the amino acid sequence deduced from ORF-B showed high homology to a putative midecamycin 4-O-methyltransferase encoded on mdmC.

MeSH terms

  • Acetyltransferases / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Carbohydrate Sequence
  • Cloning, Molecular
  • DNA, Bacterial
  • Leucomycins / biosynthesis*
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Streptomyces / enzymology
  • Streptomyces / genetics*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Leucomycins
  • carbomycin
  • Acetyltransferases
  • carbomycin 3-O-acetyltransferase

Associated data

  • GENBANK/D30759