Transformation of o-xylene to o-methyl benzoic acid by a denitrifying enrichment culture using toluene as the primary substrate

Biodegradation. 1995 Jun;6(2):141-6. doi: 10.1007/BF00695344.


A highly enriched denitrifying mixed culture transformed o-xylene co-metabolically along with toluene by methyl group oxidation. o-Methyl benzaldehyde and o-methyl benzoic acid accumulated transiently as metabolic products of o-xylene transformation. Transformation of o-methyl benzyl alcohol and o-methyl benzaldehyde occurred independently of toluene degradation and resulted in the formation of a compound coeluting with o-methyl benzoic acid on a gas chromatograph. The co-metabolic relationship between toluene and o-xylene could be attributed to a mechanism linked to the initial oxidation of the methyl group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria, Anaerobic / metabolism*
  • Benzaldehydes / chemistry
  • Benzaldehydes / metabolism
  • Biodegradation, Environmental
  • Culture Media
  • Hydrocarbons / metabolism
  • Kinetics
  • Mass Spectrometry
  • Oxidation-Reduction
  • Sewage
  • Toluene / chemistry
  • Toluene / metabolism*
  • Xylenes / chemistry
  • Xylenes / metabolism*


  • Benzaldehydes
  • Culture Media
  • Hydrocarbons
  • Sewage
  • Xylenes
  • Toluene
  • 2-xylene