Two ferritin subunits of Atlantic salmon (Salmo salar): cloning of the liver cDNAs and antibody preparation

Mol Mar Biol Biotechnol. 1995 Jun;4(2):164-70.


The ferritin heavy (H) and middle (M) subunit cDNAs were isolated from the Atlantic salmon (Salmo salar) liver. Full-length clones encoding the ferritin M subunit of 176 residues were obtained by screening of a liver cDNA library. The evolutionary conserved iron-responsive element (IRE) was identified in the upstream untranslated region. Ferritin H cDNA was cloned by running reverse transcription-polymerase chain reaction (RT-PCR) on salmon liver mRNA. The salmon ferritin H subunit of 177 residues showed 67% sequence identity with the M subunit. Northern blot analysis revealed ferritin H mRNA in the liver, gonads, head kidney, heart, and spleen, whereas M subunit mRNA was found almost exclusively in the gonads. Polyclonal antibodies against both salmon ferritin H and M were raised in rabbits.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Formation
  • Atlantic Ocean
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Female
  • Ferritins / chemistry
  • Ferritins / genetics*
  • Ferritins / immunology
  • Liver / chemistry*
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Polymerase Chain Reaction
  • Protein Conformation
  • Regulatory Sequences, Nucleic Acid / genetics
  • Salmon / genetics*
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Ferritins

Associated data

  • GENBANK/S77332
  • GENBANK/S77386