pilO, a Gene Required for Glycosylation of Pseudomonas Aeruginosa 1244 Pilin

Microbiology. 1995 May;141 ( Pt 5):1247-54. doi: 10.1099/13500872-141-5-1247.

Abstract

Nucleotide sequencing of a region downstream from the Pseudomonas aeruginosa 1244 pilin structural gene, pilA, revealed an ORF potentially able to code for a protein of M(r) 50,862. This ORF, called pilO, was flanked by a tRNAthr gene, which was followed by a transcriptional termination sequence. The tRNAthr gene and the termination sequence were nearly identical to sequences found immediately adjacent to the pilA gene of several P. aeruginosa strains. A 2200 base mRNA strand, which contained both the pilO and pilA transcripts, was produced from this region, while a 650 base transcript containing only pilA was present in a 100-fold excess over the longer transcript. Hyperexpression of the pilA gene in a PilO- strain resulted in normal pilus-specific phage sensitivity and twitching motility. The pilin produced by this strain had a lower apparent M(r) and a more neutral pl compared to that produced by a strain containing a functional pilO gene. This pilin failed to react with a sugar-specific reagent which recognized pilin produced by the strain containing a functional pilO gene.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / biosynthesis
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Base Sequence
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fimbriae Proteins
  • Fimbriae, Bacterial
  • Genes, Bacterial*
  • Genes, Regulator
  • Glycosylation
  • Molecular Sequence Data
  • Protein Processing, Post-Translational*
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / metabolism*
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • RNA, Transfer, Thr / genetics
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • DNA-Binding Proteins
  • RNA, Messenger
  • RNA, Transfer, Thr
  • Fimbriae Proteins

Associated data

  • GENBANK/X83916