Lutein associated with a transthyretin indicates carotenoid derivation and novel multiplicity of transthyretin ligands

FEBS Lett. 1995 May 22;365(1):23-6. doi: 10.1016/0014-5793(95)00389-q.


Transthyretins isolated from different species bind hydrophobic compounds and are often obtained in a yellow form. Such a transthyretin from chicken serum was purified by chromatography using Sepharose-coupled human retinol-binding protein. The yellow chromophore was extracted with methanol and purified by reverse phase HPLC followed by normal-phase chromatography on a nitrile column. Ultraviolet-visible absorbance and mass spectrometry identified the yellow compound as lutein, i.e. xanthophyll, (all-trans)-beta, epsilon-carotene-3,3'-diol, estimated to constitute 10-30% of associated colourless compounds. These components are different from the yellow component isolated from human transthyretin and establish that carotenoid-derived pigments can be associated with transthyretins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Humans
  • Ligands
  • Lutein / blood*
  • Lutein / chemistry
  • Lutein / isolation & purification
  • Prealbumin / chemistry*
  • Prealbumin / isolation & purification
  • Spectrometry, Mass, Fast Atom Bombardment
  • Spectrophotometry


  • Ligands
  • Prealbumin
  • Lutein