Effects of stilbenes isolated from medicinal plants on arachidonate metabolism and degranulation in human polymorphonuclear leukocytes

J Ethnopharmacol. 1995 Feb;45(2):131-9. doi: 10.1016/0378-8741(94)01206-f.

Abstract

Studies were made on the effects of stilbene derivatives isolated from medicinal plants on arachidonate metabolism and degranulation in human polymorphonuclear leukocytes (PMN-L). Resveratrol (3,4',5-trihydroxystilbene) isolated from the roots of Reynoutria japonica was found to inhibit the 5-lipoxygenase products 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), 5,12-dihydroxy-6,8,10,14-eicosatetraenoic acid (5,12-diHETE) and leukotriene C4(LTC4); its concentrations for 50% inhibition (IC50) were 8.90 x 10(-6) M, 6.70 x 10(-6) M and 1.37 x 10(-6) M, respectively. The IC50 of 5-HETE, 5,12-diHETE and LTC4 formations of synthetic 3,3',4-trihydroxystilbene were 5.90 x 10(-6) M, 6.30 x 10(-7) M and 8.80 x 10(-7) M, respectively. Moreover, they inhibited the release of lysosomal enzyme such as lysozyme and beta-glucuronidase induced by calcium ionophore A 23187 from human PMN-L at 10(-3)-10(-4) M.

MeSH terms

  • Arachidonic Acid / metabolism*
  • Autoradiography
  • Calcimycin / pharmacology
  • Cell Degranulation / drug effects*
  • Cyclic AMP / metabolism
  • Fatty Acids, Unsaturated / metabolism
  • Glucuronidase / metabolism
  • Humans
  • Hydroxyeicosatetraenoic Acids / antagonists & inhibitors
  • Hydroxyeicosatetraenoic Acids / metabolism
  • Leukotriene B4 / antagonists & inhibitors
  • Leukotriene B4 / metabolism
  • Leukotriene C4 / antagonists & inhibitors
  • Leukotriene C4 / metabolism
  • Lysosomes / drug effects
  • Lysosomes / enzymology
  • Muramidase / metabolism
  • Neutrophils / drug effects*
  • Plant Roots / chemistry
  • Plants, Medicinal / chemistry*
  • Resveratrol
  • Stilbenes / isolation & purification
  • Stilbenes / pharmacology*
  • Structure-Activity Relationship

Substances

  • Fatty Acids, Unsaturated
  • Hydroxyeicosatetraenoic Acids
  • Stilbenes
  • Leukotriene B4
  • Arachidonic Acid
  • Leukotriene C4
  • Calcimycin
  • 5-hydroxy-6,8,11,14-eicosatetraenoic acid
  • 12-hydroxy-5,8,10-heptadecatrienoic acid
  • 3,3',4-trihydroxystilbene
  • 15-hydroxy-5,8,11,13-eicosatetraenoic acid
  • Cyclic AMP
  • Muramidase
  • Glucuronidase
  • Resveratrol