Preparation of HeLa nuclear extracts

Methods Mol Biol. 1995:37:349-57. doi: 10.1385/0-89603-288-4:349.
No abstract available

MeSH terms

  • Binding Sites
  • Buffers
  • Cell Fractionation / methods
  • Cell Nucleus / metabolism*
  • Cell Nucleus / ultrastructure
  • Culture Techniques / methods
  • DNA-Binding Proteins / isolation & purification*
  • HeLa Cells
  • Humans
  • Indicators and Reagents
  • Sp1 Transcription Factor / isolation & purification
  • TATA Box
  • TATA-Box Binding Protein
  • Tissue Extracts / metabolism
  • Transcription Factor TFIID
  • Transcription Factors / antagonists & inhibitors
  • Transcription Factors / isolation & purification*
  • Transcription, Genetic*
  • Ultracentrifugation / methods

Substances

  • Buffers
  • DNA-Binding Proteins
  • Indicators and Reagents
  • Sp1 Transcription Factor
  • TATA-Box Binding Protein
  • Tissue Extracts
  • Transcription Factor TFIID
  • Transcription Factors