Visualization of Chromosomal Domains With Boundary Element-Associated Factor BEAF-32

Cell. 1995 Jun 16;81(6):879-89. doi: 10.1016/0092-8674(95)90008-x.

Abstract

We have purified two proteins from Drosophila that bind to the scs' boundary element of the hsp70 domain at locus 87A7. Their palindromic binding sites (CGATA-TATCG) symmetrically abut the previously mapped hypersensitive site of scs'. We have cloned a cDNA for one of these proteins, BEAF-32 (boundary element-associated factor of 32 kDa). It encodes a novel protein that is bound to scs' but not scs in vivo. Immunostaining localizes BEAF to hundreds of interbands and many puff boundaries on polytene chromosomes, suggesting that a chromosomal domain consists of a band (or puff) and part of the flanking interbands. Enhancer blocking assays implicate the palindromic binding site in boundary function. The lack of enhancer blocking in transiently transfected cells suggests an involvement of chromatin, nuclear structure, or both in boundary function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • Chromosome Banding
  • Chromosomes / metabolism
  • Chromosomes / ultrastructure*
  • Cloning, Molecular
  • DNA / genetics
  • DNA / metabolism
  • DNA, Complementary / genetics
  • Drosophila / genetics*
  • Drosophila / metabolism
  • Drosophila / ultrastructure*
  • Enhancer Elements, Genetic
  • HSP70 Heat-Shock Proteins / metabolism
  • Immunohistochemistry
  • Molecular Sequence Data
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism

Substances

  • DNA, Complementary
  • HSP70 Heat-Shock Proteins
  • Nuclear Proteins
  • DNA

Associated data

  • GENBANK/X86007