Abstract
A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensional NMR techniques revealed a high mannose type structure (Man6) with a phospho-diesterbridged N-acetylglucosamine as follows: [formula: see text]
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carbohydrate Conformation
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Carbohydrate Sequence
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Cell Line
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Chromatography, High Pressure Liquid
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Cricetinae
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Erythropoietin / biosynthesis
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Erythropoietin / chemistry*
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Glycosylation
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Humans
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Kidney
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Magnetic Resonance Spectroscopy
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Mannosephosphates / analysis*
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Mass Spectrometry
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Methylation
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Molecular Sequence Data
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Oligosaccharides / chemistry*
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Oligosaccharides / isolation & purification
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Transfection
Substances
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Mannosephosphates
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Oligosaccharides
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Recombinant Proteins
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Erythropoietin
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mannose-6-phosphate