Autoantibodies to the nucleolar organizer antigen NOR-90 in children with systemic rheumatic diseases

J Rheumatol. 1995 Mar;22(3):521-4.

Abstract

Objective: To determine the frequency of autoantibodies directed against the nucleolar organizer antigen NOR-90 [human upstream binding factor (hUBF)] in pediatric patients with systemic rheumatic diseases. Two children with antibodies to NOR-90 are reported.

Methods: Two hundred thirty-eight sera from children with systemic rheumatic diseases were screened for autoantibodies directed against hUBF by indirect immunofluorescence and immunoblotting using MOLT-4 cell extracts. Reactivity with hUBF was confirmed by immunoprecipitation of the recombinant hUBF protein.

Results: Two sera out of 238 (< 1.0%) with reactivity against hUBF were identified. One patient had recurrent abdominal pain, headache, and Raynaud's phenomenon. There has been no evidence of an active systemic rheumatic disease in more than 8 years of followup. The 2nd patient had Raynaud's phenomenon and systemic lupus erythematosus.

Conclusion: Antibodies to hUBF are rare in children with systemic rheumatic diseases. Raynaud's phenomenon was a clinical feature common to both patients.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Autoantibodies / analysis*
  • Autoantigens / immunology
  • Child
  • Child, Preschool
  • DNA-Binding Proteins / immunology*
  • Fluorescent Antibody Technique
  • Humans
  • Immunoblotting
  • Infant
  • Lupus Erythematosus, Systemic / complications
  • Lupus Erythematosus, Systemic / immunology
  • Pol1 Transcription Initiation Complex Proteins*
  • Raynaud Disease / complications
  • Raynaud Disease / immunology
  • Rheumatic Diseases / immunology*
  • Transcription Factors / immunology*

Substances

  • Autoantibodies
  • Autoantigens
  • DNA-Binding Proteins
  • Pol1 Transcription Initiation Complex Proteins
  • Transcription Factors
  • transcription factor UBF