The DNA Sequence of Equine Herpesvirus 2

J Mol Biol. 1995 Jun 9;249(3):520-8. doi: 10.1006/jmbi.1995.0314.


The complete DNA sequence of equine herpesvirus 2 (EHV-2) strain 86/67 was determined. The genome is 184,427 bp in size and has a base composition of 57.5% G + C. Unusually for a herpesvirus, about a third of the sequence distributed in several large blocks appears not to encode proteins. The 79 open reading frames that were identified as probably polypeptide-coding are predicted to encode 77 distinct proteins. Amino acid sequence comparisons confirmed that EHV-2 is a gamma-herpesvirus that is genetically collinear with herpesvirus saimiri (HVS; a gamma 2-herpesvirus) and Epstein-Barr virus (EBV; a gamma 1-herpesvirus), with a closer relationship to the former. Moreover, EHV-2 specifies eight proteins that have counterparts in HVS but not in EBV and only a single protein that has a homologue in EBV but not in HVS (EBV BCRF1, which encodes an interleukin 10-like protein). EHV-2 also encodes three potential G protein-coupled receptors, one with a counterpart in HVS that is specific for alpha chemokines, another with a counterpart in human cytomegalovirus (a beta-herpesvirus), which is specific for beta chemokines, and a third that is assigned more tentatively and lacks detectable counterparts in other herpesviruses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Composition
  • Base Sequence
  • DNA, Viral / genetics*
  • GTP-Binding Proteins / metabolism
  • Gammaherpesvirinae / genetics*
  • Genome, Viral
  • Herpesvirus 2, Saimiriine / genetics
  • Herpesvirus 4, Human / genetics
  • Horses
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Viral Proteins / genetics


  • DNA, Viral
  • Receptors, Cell Surface
  • Viral Proteins
  • GTP-Binding Proteins

Associated data

  • GENBANK/U20824