Copper, zinc superoxide dismutase in Escherichia coli: periplasmic localization

Arch Biochem Biophys. 1995 Jun 1;319(2):508-11. doi: 10.1006/abbi.1995.1324.

Abstract

Cu,ZnSOD purified from Escherichia coli has been used to raise antibodies in rabbits. The resultant antiserum was found to recognize a single band on Western blots of SDS-polyacrylamide gel electropherograms, and that single band coincided with the position of the Cu,ZnSOD. Ultrathin sections of fixed E. coli were treated with the antibody followed by protein A bearing 10-nm gold particles. Electron microscopy revealed that Cu,ZnSOD was largely localized in the periplasm in polar bays.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies
  • Copper / chemistry
  • Escherichia coli / enzymology*
  • Escherichia coli / ultrastructure
  • Immunohistochemistry
  • Microscopy, Electron
  • Rabbits
  • Superoxide Dismutase / analysis
  • Superoxide Dismutase / chemistry
  • Superoxide Dismutase / isolation & purification*
  • Zinc / chemistry

Substances

  • Antibodies
  • Copper
  • Superoxide Dismutase
  • Zinc