Abstract
A new 8.2-kDa protein factor was isolated from cell culture of a promyelocyte leukemic HL-60 cell line that exhibited a differentiation-inducing effect upon the initial cell line. The primary structure of this factor was determined by sequencing the protein (N-terminal region) and the corresponding cDNA. A molecule of the differentiation factor secreted by HL-60 cells consisted of 54 amino acid residues and was glycosylated.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Differentiation
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Chromatography, Gel
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Cloning, Molecular
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Culture Media
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DNA, Complementary
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Glycosylation
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Humans
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Molecular Sequence Data
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Neoplasm Proteins / genetics
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Neoplasm Proteins / isolation & purification*
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Neoplasm Proteins / metabolism
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Tretinoin / pharmacology*
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Tumor Cells, Cultured
Substances
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Culture Media
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DNA, Complementary
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Neoplasm Proteins
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cell differentiation factor 8.2-kDa
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Tretinoin