Fluorescence lifetime-based sensing in tissues: a computational study

Biophys J. 1995 Apr;68(4):1574-82. doi: 10.1016/S0006-3495(95)80330-9.


We have numerically solved the photon diffusion equation to predict the distribution of light in a tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biophysical Phenomena
  • Biophysics
  • Biosensing Techniques
  • Diffusion
  • Fluorescence*
  • Fluorescent Dyes / pharmacokinetics*
  • Light
  • Models, Biological
  • Photons
  • Scattering, Radiation
  • Tissue Distribution


  • Fluorescent Dyes