Structural organization and differential expression of carrot beta-fructofuranosidase genes: identification of a gene coding for a flower bud-specific isozyme

Plant Mol Biol. 1995 Apr;28(1):189-94. doi: 10.1007/BF00042049.

Abstract

Three genomic clones (Inv*Dc1, Inv*Dc2 and Inv*Dc3) were isolated by using the cDNA for carrot cell wall beta-fructofuranosidase as a probe. The expression patterns of the three genes differed markedly. High levels of Inv*Dc1 transcripts were found in leaves and roots of young carrot, whereas in plants with developing tap roots no transcripts were detected. A high level of mRNA of Inv*Dc1 was also present in suspension-cultured cells. In developing reproductive organs, only low levels of transcripts of Inv*Dc1 were found in flower buds and flowers and none at later stages of development. In contrast, Inv*Dc2 and Inv*Dc3 were not expressed in vegetative plant organs. Invb1*Dc1 was exclusively and strongly expressed in flower buds, and Inv*Dc3 at a very low level in suspension-cultured cells.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Chromosome Mapping
  • Daucus carota / enzymology
  • Daucus carota / genetics*
  • Daucus carota / growth & development
  • Genes, Plant / genetics*
  • Glycoside Hydrolases / biosynthesis
  • Glycoside Hydrolases / genetics*
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics*
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • beta-Fructofuranosidase

Substances

  • Isoenzymes
  • Glycoside Hydrolases
  • beta-Fructofuranosidase

Associated data

  • GENBANK/X69321
  • GENBANK/X78423
  • GENBANK/X78424