Molecular cloning of a cDNA for proctase B from Aspergillus niger var. macrosporus and sequence comparison with other aspergillopepsins I

Biosci Biotechnol Biochem. 1995 May;59(5):954-5. doi: 10.1271/bbb.59.954.

Abstract

A cDNA for proctase B from Aspergillus niger var. macrosporus was isolated and sequenced. The deduced amino acid sequence (394 residues) of the preproform of the enzyme was highly homologous (98% identify) with those of aspergillopepsins I from A. awamori and A. saitoi, and moderately homologous (68% identity) with that of A. oryzae. Most of the sequence differences were found in the carboxyl-terminal domain.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Aspartic Acid Endopeptidases / chemistry
  • Aspartic Acid Endopeptidases / genetics*
  • Aspergillus niger / enzymology*
  • Aspergillus niger / genetics
  • Base Sequence
  • Cloning, Molecular*
  • DNA, Complementary / chemistry
  • Molecular Sequence Data
  • Protein Conformation
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Structure-Activity Relationship

Substances

  • DNA, Complementary
  • Aspartic Acid Endopeptidases
  • aspergillopepsin I

Associated data

  • GENBANK/D45177