Abstract
A cDNA for proctase B from Aspergillus niger var. macrosporus was isolated and sequenced. The deduced amino acid sequence (394 residues) of the preproform of the enzyme was highly homologous (98% identify) with those of aspergillopepsins I from A. awamori and A. saitoi, and moderately homologous (68% identity) with that of A. oryzae. Most of the sequence differences were found in the carboxyl-terminal domain.
MeSH terms
-
Amino Acid Sequence
-
Aspartic Acid Endopeptidases / chemistry
-
Aspartic Acid Endopeptidases / genetics*
-
Aspergillus niger / enzymology*
-
Aspergillus niger / genetics
-
Base Sequence
-
Cloning, Molecular*
-
DNA, Complementary / chemistry
-
Molecular Sequence Data
-
Protein Conformation
-
Sequence Homology, Amino Acid
-
Sequence Homology, Nucleic Acid
-
Structure-Activity Relationship
Substances
-
DNA, Complementary
-
Aspartic Acid Endopeptidases
-
aspergillopepsin I