Quantitative analysis of the lens cell microstructure in selenite cataract using a two-dimensional Fourier analysis

Exp Eye Res. 1995 Mar;60(3):245-55. doi: 10.1016/s0014-4835(05)80107-1.


Using two-dimensional (2-D) Fourier methods, we analysed the cellular microstructure of three rat lenses: normal transparent, selenite-induced cataractous and selenite-treated plus a phase separation inhibitor (PSI) to prevent cataract. 2-D Fourier analysis of electron micrographs of the lens cells quantified the dimensions of the spatial fluctuations in electron density of the lens cell microstructure. The 2-D Fourier spectra of the transparent normal and PSI-treated lens cells were remarkably similar while those of the opaque selenite-treated lens cells were dramatically different. In the opaque cells the contributions of large Fourier components (larger than half the wavelength of light) in the 2-D Fourier spectra were much greater than in the transparent cells. The results of the 2-D Fourier analysis of electron micrographs are consistent with the theory of transparency of the eye.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amifostine / analogs & derivatives
  • Amifostine / pharmacology
  • Animals
  • Cataract / chemically induced
  • Cataract / pathology*
  • Fourier Analysis*
  • Image Processing, Computer-Assisted
  • Lens, Crystalline / ultrastructure*
  • Microscopy, Electron
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Selenite / antagonists & inhibitors


  • WR 77913
  • Sodium Selenite
  • Amifostine