Isolation and Characterization of ompS1, a Novel Salmonella Typhi Outer Membrane Protein-Encoding Gene

Gene. 1995 May 26;158(1):67-72. doi: 10.1016/0378-1119(95)00171-2.

Abstract

We have isolated a novel outer membrane protein (OMP)-encoding gene from Salmonella typhi (St), termed ompS1, using the ompF gene of Escherichia coli (Ec) as a heterologous probe. The structural ompS1 gene codes for an OmpS1 polypeptide that consists of 373 amino acids (aa) in the mature product, with a putative 21-aa leader sequence, containing highly conserved aa residues that have been implicated in pore formation. Mature OmpS1 (41 kDa) is larger than the OmpC, OmpF and PhoE St and Ec porins. In contrast to the major porins, it is undetectable in Coomassie-stained OMP preparations; although, when ompS1 was cloned into a high-copy-number plasmid under the control of the inducible tac promoter, it was detectable along with major OMPs. The 5' regulatory region of ompS1 has five putative binding sites for OmpR, a positive transcriptional regulator. The ompS1 gene shows restriction-fragment length polymorphism (RFLP) among Salmonellae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / genetics*
  • Base Sequence
  • DNA, Bacterial / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Porins
  • Regulatory Sequences, Nucleic Acid
  • Salmonella typhi / genetics*
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Outer Membrane Proteins
  • DNA, Bacterial
  • OmpS1 protein, Salmonella typhi
  • Porins

Associated data

  • GENBANK/X73237