ILR1, an amidohydrolase that releases active indole-3-acetic acid from conjugates

Science. 1995 Jun 23;268(5218):1745-8. doi: 10.1126/science.7792599.

Abstract

In plants, the growth regulator indole-3-acetic acid (IAA) is found both free and conjugated to a variety of amino acids, peptides, and carbohydrates. IAA conjugated to leucine has effects in Arabidopsis thaliana similar to those of free IAA. The ilr1 mutant is insensitive to exogenous IAA-Leu and was used to positionally clone the Arabidopsis ILR1 gene. ILR1 encodes a 48-kilodalton protein that cleaves IAA-amino acid conjugates in vitro and is homologous to bacterial amidohydrolase enzymes. DNA sequences similar to that of ILR1 are found in other plant species.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amidohydrolases / chemistry
  • Amidohydrolases / genetics*
  • Amidohydrolases / metabolism
  • Amino Acid Sequence
  • Amino Acids
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Cloning, Molecular
  • Genes, Plant*
  • Hydrolysis
  • Indoleacetic Acids / metabolism*
  • Indoleacetic Acids / pharmacology
  • Leucine / metabolism
  • Molecular Sequence Data
  • Mutation
  • Plant Growth Regulators / metabolism*
  • Sequence Alignment

Substances

  • Amino Acids
  • Arabidopsis Proteins
  • Indoleacetic Acids
  • Plant Growth Regulators
  • indoleacetic acid
  • Amidohydrolases
  • ILR1 protein, Arabidopsis
  • Leucine

Associated data

  • GENBANK/U23794
  • GENBANK/U23795
  • GENBANK/U23796
  • GENBANK/U53221