Multiple Vibrio vulnificus strains in oysters as demonstrated by clamped homogeneous electric field gel electrophoresis

C Buchrieser; V V Gangar; R L Murphree; M L Tamplin; C W Kaspar

doi:10.1128/aem.61.3.1163-1168.1995

Multiple Vibrio vulnificus strains in oysters as demonstrated by clamped homogeneous electric field gel electrophoresis

Appl Environ Microbiol. 1995 Mar;61(3):1163-8. doi: 10.1128/aem.61.3.1163-1168.1995.

Authors

C Buchrieser¹, V V Gangar, R L Murphree, M L Tamplin, C W Kaspar

Affiliation

¹ Food Research Institute, University of Wisconsin, Madison 53706.

Abstract

Clamped homogeneous electric field gel electrophoresis and a computer program for managing electrophoresis banding patterns (ELBAMAP) were used to analyze genomic DNA of 118 Vibrio vulnificus strains, isolated from three oysters by direct plating. Analysis with SfiI resulted in 60 restriction endonuclease digestion profiles (REDP), while analysis with SrfI produced 53 different REDP. Similarities between REDP ranged from 7 to 93%. Principal-component analysis showed that the strains were heterogeneous.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Base Sequence
DNA, Bacterial / analysis
DNA, Bacterial / genetics*
Electrophoresis, Gel, Pulsed-Field / methods*
Molecular Sequence Data
Ostreidae / microbiology*
Polymorphism, Restriction Fragment Length
Software
Vibrio / genetics
Vibrio / isolation & purification*

Substances

DNA, Bacterial