Human proadrenomedullin N-terminal 20 peptide in pheochromocytoma and normal adrenal medulla

Biochem Biophys Res Commun. 1995 Jun 15;211(2):694-9. doi: 10.1006/bbrc.1995.1867.

Abstract

Proadrenomedullin N-terminal 20 peptide (PAMP) is a novel hypotensive peptide found in adrenomedullin precursor. Using a radioimmunoassay for human PAMP, we purified immunoreactive PAMP (ir-PAMP) from human pheochromocytoma and determined its complete amino acid sequence. The major component of PAMP-like immunoreactivity was found to be PAMP [1-20] NH2 with an amino acid sequence identical to that of the deduced amino acid sequence by cDNA analysis. Both ir-PAMP and ir-adrenomedullin were found to be abundant in normal adrenal medulla as well as pheochromocytoma tissue arising from adrenal medulla, and there was a significantly (p < 0.05) positive correlation between ir-adrenomedullin and ir-PAMP concentrations in these tissues. However, the PAMP/adrenomedullin ratio in pheochromocytoma tissues (0.197 +/- 0.013) was significantly (p < 0.005) lower than that in adrenal medullae (0.384 +/- 0.041). The present data indicate that PAMP is biosynthesized from adrenomedullin precursor, but the biosynthesis or metabolism of PAMP in pheochromocytoma may be different from that of normal adrenal medulla.

Publication types

  • Comparative Study

MeSH terms

  • Adrenal Gland Neoplasms / chemistry*
  • Adrenal Gland Neoplasms / metabolism
  • Adrenal Medulla / chemistry*
  • Adrenal Medulla / metabolism
  • Adrenomedullin
  • Amino Acid Sequence
  • Antihypertensive Agents*
  • Cadaver
  • Humans
  • Molecular Sequence Data
  • Peptide Biosynthesis
  • Peptide Fragments / analysis*
  • Peptide Fragments / chemistry
  • Peptides / analysis*
  • Pheochromocytoma / chemistry*
  • Pheochromocytoma / metabolism
  • Protein Precursors / analysis*
  • Protein Precursors / metabolism
  • Proteins / analysis*
  • Radioimmunoassay

Substances

  • Antihypertensive Agents
  • Peptide Fragments
  • Peptides
  • Protein Precursors
  • Proteins
  • Adrenomedullin