To study the importance of site of recovery and other factors related to mobile-bag (MB) digestion values, two consecutive experiments in which diets were applied in a 3 x 3 Latin square design, were carried out with cannulated growing heifers. In Expt 1, several types of experimental feed were exposed to intestinal digestion in mobile bags made of two cloth types and filled with intact or rumen-undegradable (RUD) feed material to be recovered either from the ileum (IB) or faeces (FB). In Expt 2, mean retention time (MRT) of Yb-labelled digesta particles within the intestine and in vivo digestibility of diets were measured. With vegetable concentrates, FB resulted generally in slight overestimation of small-intestinal dry matter and N digestion, while with meat-and-bone meal no difference between FB and IB was found. The respective N digestibility of RUD late-cut silage was clearly underestimated as measured from FB. The disappearance of neutral-detergent fibre (NDF) of all feeds under test was higher from FB than from IB. It was not possible to isolate the influence of the large intestine on the MB values by changing bag cloth type. Irrespective of the longer retention time of bags and longer MRT of Yb in the intestine on a low as compared with a high level of feeding, only NDF disappearance of feeds increased due to lower feeding level. Altering the diet type to increase large-intestinal fermentation, as indicated in vivo, usually had no effect on the MB values. It is concluded that the site of collection of bags does not practically affect small-intestinal digestion values of feed N, unless the feed is rich in fibre.