Oral immunization with Helicobacter pylori urease B subunit as a treatment against Helicobacter infection in mice

Gastroenterology. 1995 Jul;109(1):115-21. doi: 10.1016/0016-5085(95)90275-9.


Background & aims: Eradication of Helicobacter pylori infections in humans results in the healing of gastritis and gastric ulcers. This study used a mouse model to test whether oral vaccination can cure Helicobacter infection and gastritis.

Methods: Mice were infected with Helicobacter felis. Three weeks after infection, the mice were orally immunized with H. pylori urease B subunit. Control mice were simultaneously infected but sham immunized.

Results: Three to 8 weeks after oral immunization of H. felis-infected mice with recombinant H. pylori urease B subunit, the infection cleared and there was no evidence of gastritis. Vaccinated mice remained protected against two consecutive H. felis challenges.

Conclusions: These results show that the lack of natural immunity against Helicobacter can be overcome by oral immunization and that vaccination offers a novel therapeutic approach to Helicobacter-induced gastritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Disease Models, Animal
  • Female
  • Helicobacter Infections / immunology
  • Helicobacter Infections / prevention & control
  • Helicobacter Infections / therapy*
  • Helicobacter pylori / enzymology*
  • Immunotherapy*
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / therapeutic use
  • Recurrence
  • Urease / administration & dosage
  • Urease / therapeutic use*


  • Recombinant Proteins
  • Urease