Replication of influenza A viruses in a green monkey kidney continuous cell line (Vero)

J Infect Dis. 1995 Jul;172(1):250-3. doi: 10.1093/infdis/172.1.250.


A Vero cell line was investigated as a suitable host system for primary isolation and cultivation of influenza A viruses. The efficiency of primary isolation for currently circulating (H3N2) strains was similar in Vero and MDCK cells. Of 72 egg-adapted strains investigated, 90.3% were detectable hy hemagglutinin (HA) titration in Vero cells after the first passage and 51.4% after the second. The amino acid sequences of the HA1 region of influenza A viruses isolated and passaged in Vero cells were identical to those of their MDCK-grown counterparts. At low MOI, high yields of influenza virus were achieved in Vero cells by multiple additions of trypsin to the medium. After 20 passages of A/England/1/53 (H1N1) in Vero cells, the titer of infectious virus was 8.37 log10 TCID50/mL, and virus protein yields were as high as in MDCK cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Child
  • Chlorocebus aethiops
  • Dogs
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral / analysis
  • Hemagglutinins, Viral / chemistry
  • Humans
  • Influenza A virus / isolation & purification
  • Influenza A virus / physiology*
  • Influenza, Human / virology
  • Kidney
  • Polymerase Chain Reaction
  • RNA, Viral / analysis
  • Vero Cells
  • Viral Envelope Proteins / analysis
  • Viral Plaque Assay
  • Virus Replication*


  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral
  • RNA, Viral
  • Viral Envelope Proteins