Coagulation of whole blood stimulates interleukin-1 beta gene expression

J Infect Dis. 1995 Jul;172(1):308-11. doi: 10.1093/infdis/172.1.308.


To study interleukin (IL)-1 beta gene expression, reverse transcription-polymerase chain reaction was used on 25-microL whole blood samples from 11 healthy subjects. Coagulated and unclotted whole blood was compared. There was no evidence of IL-1 beta gene expression in any time zero samples (i.e., whole blood from which mRNA was immediately extracted) from 11 subjects, whereas a 388-bp band representing IL-1 beta mRNA was detected in all coagulated samples. No mRNA for IL-1 beta was detected in EDTA-anticoagulated whole blood, although in these samples the addition of lipopolysaccharide as a positive control induced the expression of IL-1 beta. In time course studies on samples allowed to clot, mRNA for IL-1 beta was detectable after 30 min. These findings demonstrate that IL-1 beta gene expression is not present in circulating cells of healthy subjects and that coagulation is a stimulus for IL-1 beta gene expression. This may be a mechanism by which thrombosis produces inflammation and fever.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Base Sequence
  • Blood Coagulation / physiology*
  • DNA Primers
  • Edetic Acid / pharmacology
  • Female
  • Gene Expression* / drug effects
  • Humans
  • In Vitro Techniques
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / blood
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis


  • DNA Primers
  • Interleukin-1
  • RNA, Messenger
  • Edetic Acid