Rapid detection of virus genome from imported dengue fever and dengue hemorrhagic fever patients by direct polymerase chain reaction

J Med Virol. 1994 Sep;44(1):54-8. doi: 10.1002/jmv.1890440111.


Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis of dengue infection.

MeSH terms

  • Adult
  • Base Sequence
  • Child, Preschool
  • Dengue / virology*
  • Dengue Virus / classification
  • Dengue Virus / genetics
  • Dengue Virus / isolation & purification*
  • Female
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Serotyping
  • Time Factors
  • Travel