In vitro steroidogenic effects of neuropeptide Y (NPY1-36), Y1 and Y2 receptor agonists (Leu31-Pro34 NPY, NPY18-36) and peptide YY (PYY) on rat adrenal capsule/zona glomerulosa

Regul Pept. 1994 Aug 4;52(3):187-93. doi: 10.1016/0167-0115(94)90053-1.


The effects of neuropeptide Y (NPY1-36), of two analogs (Leu31-Pro34 NPY and NPY18-36) and of Peptide YY (PYY) on aldosterone and corticosterone secretions by freshly isolated rat adrenal capsule/zona glomerulosa preparations were investigated in vitro. NPY-related peptides (NPY1-36, Leu31-Pro34 NPY, NPY18-36), but not PYY, induced a dose-dependent release of aldosterone at concentrations ranging from 10(-8) to 10(-6) M. All the investigated peptides failed to significantly affect corticosterone secretion in concentrations ranging from 10(-10) to 10(-6) M (NPY1-36, NPY18-36), 10(-11) to 10(-6) M (Leu31-Pro34 NPY) or 10(-9) to 10(-6) M (PYY). Aldosterone secretion by this preparation of isolated adrenal capsule/zona glomerulosa was also significantly stimulated by high potassium levels (55 mEq) or by angiotensin II (AII) in concentrations ranging from 10(-8) to 10(-6) M. Moreover, NPY and Y1 or Y2 receptor agonists were positive aldosterone releasing agents as potent as AII. The present data support the existence of: (1) NPY binding sites of the Y3-like subtype, on rat adrenal capsule/zona glomerulosa. (2) A stimulatory effect of NPY on aldosterone production. So that the NPYergic innervation of the rat adrenal capsule/zona glomerulosa could be implicated in the multifactorial control of aldosterone production.

MeSH terms

  • Aldosterone / biosynthesis*
  • Aldosterone / metabolism
  • Angiotensin II / pharmacology
  • Animals
  • Corticosterone / biosynthesis*
  • Corticosterone / metabolism
  • Male
  • Neuropeptide Y / analogs & derivatives
  • Neuropeptide Y / pharmacology*
  • Organ Size / drug effects
  • Peptide Fragments
  • Peptide YY
  • Peptides / pharmacology*
  • Potassium / pharmacology
  • Rats
  • Rats, Wistar
  • Zona Glomerulosa / drug effects*
  • Zona Glomerulosa / metabolism


  • Neuropeptide Y
  • Peptide Fragments
  • Peptides
  • Peptide YY
  • Angiotensin II
  • neuropeptide Y (18-36)
  • neuropeptide Y, Leu(31)-Pro(34)-
  • Aldosterone
  • Potassium
  • Corticosterone