Hepatocyte growth factor region specifically stimulates gastro-intestinal epithelial growth in primary culture

Biochem Biophys Res Commun. 1994 Dec 15;205(2):1445-51. doi: 10.1006/bbrc.1994.2827.


Hepatocyte growth factor (HGF) stimulated the growth of fetal rat gastrointestinal epithelial cells in primary culture with a clear dose-response relationship. The epithelial response to the mitogenic activity of HGF was different among the region of the gastro-intestinal tract; glandular stomach most responsive, followed by intestine and forestomach. The interaction of HGF with other growth factors in inducing the epithelial growth was also different depending on the type of the epithelial cells, indicating a region-specific growth regulation in the gastro-intestinal tract. Analyses using Northern blot and RT-PCR revealed that HGF mRNA was expressed only in mesenchymes but not in epithelia of the gastro-intestinal tract while expression of c-met (HGF receptor) gene was observed in both tissues. These results suggest that gastro-intestinal mesenchymes secrete HGF which stimulates the growth of attaching epithelial cells by a paracrine mechanism, and that the epithelial response to HGF is controlled by a region-specific growth regulatory mechanism.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Division / drug effects*
  • Cells, Cultured
  • DNA Primers
  • Epithelial Cells
  • Epithelium / drug effects
  • Fetus
  • Gastric Mucosa / metabolism
  • Growth Substances / pharmacology
  • Hepatocyte Growth Factor / biosynthesis*
  • Hepatocyte Growth Factor / pharmacology*
  • Humans
  • Intestinal Mucosa / metabolism
  • Intestines / cytology*
  • Intestines / drug effects
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / biosynthesis
  • Proto-Oncogene Proteins c-met
  • Rats
  • Rats, Inbred F344
  • Receptor Protein-Tyrosine Kinases / biosynthesis*
  • Recombinant Proteins / pharmacology
  • Stomach / cytology*
  • Stomach / drug effects


  • DNA Primers
  • Growth Substances
  • Proto-Oncogene Proteins
  • Recombinant Proteins
  • Hepatocyte Growth Factor
  • Proto-Oncogene Proteins c-met
  • Receptor Protein-Tyrosine Kinases