Sequencing of xyloglucan oligosaccharides by partial Driselase digestion: the preparation and quantitative and qualitative analysis of two new tetrasaccharides

Carbohydr Res. 1994 Oct 17;263(2):285-93. doi: 10.1016/0008-6215(94)00170-7.

Abstract

The pentasaccharide (XXG), [formula: see text] obtained from Rosa xyloglucan, was converted to two isomeric tetrasaccharides, a and b (Xyl1.Glc3), by mild acid hydrolysis. During hydrolysis in 2 M trifluoroacetic acid at 90 degrees C, optimal yields of a and b were obtained after 20-40 min. Each tetrasaccharide was purified by preparative paper chromatography and high-pressure liquid chromatography (HPLC). The two isomers were distinguished by the products of their partial digestion with Driselase, which hydrolyses the glucosidic bonds sequentially from the non-reducing terminus: a and b yielded cellobiose and Xyl-->Glc-->Glc, respectively showing that they were [formula: see text] and [formula: see text] respectively. Tetrasaccharide b was chromatographically identical, upon HPLC on Dionex CarboPac PA1, with the tetrasaccharide produced from XXG by the action of Tropaeolum alpha-D-xylosidase, supporting the proposed structure. Xyloglucan oligosaccharides were assayed quantitatively by measurement of the yield of isoprimeverose (Xyl-->Glc) after complete Driselase digestion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Chromatography, Paper
  • Fungal Proteins*
  • Glucans*
  • Glycoside Hydrolases / metabolism*
  • Hydrolysis
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Polyporaceae / enzymology
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Xylans*

Substances

  • Fungal Proteins
  • Glucans
  • Oligosaccharides
  • Polysaccharides
  • Xylans
  • xyloglucan
  • Glycoside Hydrolases
  • driselase