Possible involvement of the 440 kDa isoform of ankyrinB in neuritogenesis in human neuroblastoma NB-1 cells

FEBS Lett. 1995 Jan 3;357(2):217-20. doi: 10.1016/0014-5793(94)01363-6.


Two isoforms of brain ankyrin, 440 kDa and 220 kDa ankyrinB, which are products of alternatively spliced pre-mRNA encoded by a single gene, are both expressed in human neuroblastoma NB-1 cells. Expression of the polypeptide and mRNA of the larger isoform increased upon induction of neurite outgrowth in NB-1 cells by dibutyryl cAMP, while those of the smaller isoform were unaffected. The expressed 440 kDa ankyrinB was concentrated at the tip of growing neurites and was partly co-localized with GAP-43. These results suggest that 440 kDa ankyrinB is one of the neuronal growth-associated proteins and provides an interesting example of gene regulation by alternative splicing in neuronal cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Ankyrins / chemistry
  • Ankyrins / genetics
  • Ankyrins / physiology*
  • Brain / physiology
  • Bucladesine / pharmacology
  • Cell Differentiation
  • Humans
  • Neurites / drug effects
  • Neurites / physiology*
  • Neuroblastoma
  • Stereoisomerism
  • Tumor Cells, Cultured


  • Ankyrins
  • Bucladesine