The present study assesses the relationship between self-reported dental anxiety (Dental Anxiety Inventory, Dental Anxiety Scale, and Duration of Psychophysiological Fear Reactions), electrodermal activity (skin-conductance level and frequency of spontaneous responses), and Marlowe-Crowne defensiveness. All measurements were made twice. The first session was scheduled immediately before a semi-annual dental check-up (stress condition), and baseline measurements were made two months later without the prospect of a dental appointment. Subjects were male dental patients who regularly attended a university dental clinic and a clinic for Special Dental Care. The main findings were that the low anxious-high defensive-scoring (Marlowe-Crowne Denial subscale) university patients showed significantly higher skin-conductance levels and frequency of nonspecific fluctuations than the low anxious-low defensive-scoring subjects. Besides, the conductance values of the low anxious-high defensive-scoring subjects resembled those of the high anxious-low defensive-scoring patients of the clinic for Special Dental Care, the baseline frequency of nonspecific fluctuations excepted.