Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases

Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12448-52. doi: 10.1073/pnas.91.26.12448.


We report the characterization of a member of the ras gene family that is overexpressed in cells transformed by abl tyrosine kinase oncogenes. The gene, named kir (for kinase-inducible ras-like), is induced at the transcriptional level. kir mRNA has a rapid turnover and encodes a protein of 33 kDa with guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. kir was cloned by differential screening of genes present in fully malignant versus growth factor-independent cell lines expressing wild-type or mutant forms of BCR/ABL. BCR/ABL and v-Abl induce transcription of the kir gene via specific signaling pathway(s), but kir overexpression alone is not sufficient to mediate transformation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Cell Transformation, Neoplastic*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Fusion Proteins, bcr-abl / metabolism*
  • GTP-Binding Proteins / genetics*
  • GTP-Binding Proteins / metabolism
  • Gene Expression Regulation, Neoplastic*
  • Immediate-Early Proteins / genetics*
  • Immediate-Early Proteins / metabolism
  • Mice
  • Molecular Sequence Data
  • Monomeric GTP-Binding Proteins*
  • Oncogene Proteins v-abl / metabolism*
  • Protein-Tyrosine Kinases / metabolism*
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Transcription, Genetic


  • DNA, Complementary
  • Immediate-Early Proteins
  • Oncogene Proteins v-abl
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Fusion Proteins, bcr-abl
  • GTP-Binding Proteins
  • Gem protein, mouse
  • Monomeric GTP-Binding Proteins

Associated data

  • GENBANK/U13052
  • GENBANK/U13053