Molecular cloning and functional characterization of a human VIP receptor from SUP-T1 lymphoblasts

Biochem Biophys Res Commun. 1994 Dec 30;205(3):1617-24. doi: 10.1006/bbrc.1994.2852.


We have cloned and sequenced a cDNA isolated from a human SUP-T1 lymphoblast cell line library. It encoded a 457 amino acids protein having 87% identity with the rat PACAP type II, VIP2 receptor. Chinese hamster ovary (CHO) cells stably transfected with cloned cDNA expressed a specific binding of 125I[Acetyl-His1]PACAP-27. This binding was inhibited by GTP, and by the peptides helodermin, VIP, PACAP-27 and PACAP-38 that also stimulated adenylate cyclase activity. The order of potency was PACAP-38 > VIP > or = helodermin > or = PACAP-27. Comparison of the results in two cell lines expressing different receptor densities suggested that helodermin and PACAP-38 had a higher intrinsic activity than VIP and PACAP-27.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Lymphocytes / metabolism*
  • Molecular Sequence Data
  • Neuropeptides / metabolism
  • Peptides / metabolism
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Rats
  • Receptors, Vasoactive Intestinal Peptide / genetics*
  • Receptors, Vasoactive Intestinal Peptide / metabolism
  • Receptors, Vasoactive Intestinal Peptide, Type II
  • Sequence Homology, Amino Acid
  • Transfection
  • Vasoactive Intestinal Peptide / metabolism


  • ADCYAP1 protein, human
  • Adcyap1 protein, rat
  • DNA Primers
  • DNA, Complementary
  • Intercellular Signaling Peptides and Proteins
  • Neuropeptides
  • Peptides
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Receptors, Vasoactive Intestinal Peptide
  • Receptors, Vasoactive Intestinal Peptide, Type II
  • VIPR2 protein, human
  • Vasoactive Intestinal Peptide
  • heliodermin

Associated data

  • GENBANK/L36566
  • GENBANK/L40764