Sequence of the 5'-flanking region of CYP3A5: comparative analysis with CYP3A4 and CYP3A7

Biochem Biophys Res Commun. 1994 Dec 30;205(3):1741-7. doi: 10.1006/bbrc.1994.2870.


Two genomic clones containing the 3'-end of CYP3A7, the whole 5'-flanking region plus the first exon of CYP3A5 were isolated from two different human genomic banks and sequenced. The 5'-flanking region of CYP3A5, from nucleotide -1 to -1434, was 60% and 59% similar with the corresponding regions of CYP3A4 and CYP3A7, respectively. However, the similarity increased to 74% when regions from nucleotide -1 to -700 were compared and dropped to less than 42% for regions upstream from -700. The CYP3A5 promoter contains a CATA box instead of the typical TATA box and a basic transcription element (BTE). Other consensus sequences previously found in CYP3A4 and CYP3A7 were also identified in the 5'-flanking of CYP3A5 from -1 to -700. Both CYP3A5 and CYP3A7 genes appear to be tandemly associated and transcribed in the same direction on chromosome 7.

Publication types

  • Comparative Study

MeSH terms

  • Aryl Hydrocarbon Hydroxylases*
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7
  • Cloning, Molecular
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA, Complementary / genetics
  • Humans
  • Molecular Sequence Data
  • Multigene Family
  • Oxidoreductases, N-Demethylating / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • DNA, Complementary
  • Cytochrome P-450 Enzyme System
  • Aryl Hydrocarbon Hydroxylases
  • CYP3A7 protein, human
  • Cytochrome P-450 CYP3A
  • Oxidoreductases, N-Demethylating

Associated data

  • GENBANK/S74699
  • GENBANK/S74700